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    A Radiation Heating-Based Oscillatory Polymerase Chain Reaction System for Detecting Donkey-Hide Gelatin

    Source: Journal of Thermal Science and Engineering Applications:;2025:;volume( 017 ):;issue: 003::page 31005-1
    Author:
    Chen, Jyh Jian
    ,
    Kuo, Tsung Yueh
    ,
    Sheu, Shyang Chwen
    ,
    Song, Yi Syuan
    DOI: 10.1115/1.4067486
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: Polymerase chain reaction (PCR) is a biochemical technique for copying DNA by repeatedly changing the temperature of nucleic acid samples. In this study, we aim to create an oscillatory PCR system with a short reaction time, which could have significant practical implications. The device uses an electromechanical module with a servo motor and a homemade heating–cooling system that combines a halogen lamp, a Peltier element, a cooling fin, and a blower fan. We code the motor program to control the reaction chamber moving back and forth in the infrared thermal cycling system. The system uses one infrared lamp for heating and one Peltier element/thermal dissipation fins/blower fan for cooling to shorten the overall reaction time of the thermal process. Results show that using the radiant heating and convection cooling method and a micro-sample of 10 μL to perform a PCR, the total time spent is 35 min, which saves about 1 h compared to commercially available PCR instruments. The proposed PCR approach could specifically detect donkey-hide gelatin (DHG) made from donkey skin, offering a rapid and cost-effective solution. Therefore, our device has the advantages of easy manufacturing, low cost, and rapid temperature ramping rate for PCR.
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      A Radiation Heating-Based Oscillatory Polymerase Chain Reaction System for Detecting Donkey-Hide Gelatin

    URI
    http://yetl.yabesh.ir/yetl1/handle/yetl/4306246
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    • Journal of Thermal Science and Engineering Applications

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    contributor authorChen, Jyh Jian
    contributor authorKuo, Tsung Yueh
    contributor authorSheu, Shyang Chwen
    contributor authorSong, Yi Syuan
    date accessioned2025-04-21T10:27:45Z
    date available2025-04-21T10:27:45Z
    date copyright1/16/2025 12:00:00 AM
    date issued2025
    identifier issn1948-5085
    identifier othertsea_17_3_031005.pdf
    identifier urihttp://yetl.yabesh.ir/yetl1/handle/yetl/4306246
    description abstractPolymerase chain reaction (PCR) is a biochemical technique for copying DNA by repeatedly changing the temperature of nucleic acid samples. In this study, we aim to create an oscillatory PCR system with a short reaction time, which could have significant practical implications. The device uses an electromechanical module with a servo motor and a homemade heating–cooling system that combines a halogen lamp, a Peltier element, a cooling fin, and a blower fan. We code the motor program to control the reaction chamber moving back and forth in the infrared thermal cycling system. The system uses one infrared lamp for heating and one Peltier element/thermal dissipation fins/blower fan for cooling to shorten the overall reaction time of the thermal process. Results show that using the radiant heating and convection cooling method and a micro-sample of 10 μL to perform a PCR, the total time spent is 35 min, which saves about 1 h compared to commercially available PCR instruments. The proposed PCR approach could specifically detect donkey-hide gelatin (DHG) made from donkey skin, offering a rapid and cost-effective solution. Therefore, our device has the advantages of easy manufacturing, low cost, and rapid temperature ramping rate for PCR.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleA Radiation Heating-Based Oscillatory Polymerase Chain Reaction System for Detecting Donkey-Hide Gelatin
    typeJournal Paper
    journal volume17
    journal issue3
    journal titleJournal of Thermal Science and Engineering Applications
    identifier doi10.1115/1.4067486
    journal fristpage31005-1
    journal lastpage31005-10
    page10
    treeJournal of Thermal Science and Engineering Applications:;2025:;volume( 017 ):;issue: 003
    contenttypeFulltext
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    DSpace software copyright © 2002-2015  DuraSpace
    نرم افزار کتابخانه دیجیتال "دی اسپیس" فارسی شده توسط یابش برای کتابخانه های ایرانی | تماس با یابش
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