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    Measurement of the Water Permeability of Single Human Granulocytes on a Microscopic Stopped-Flow Mixing System

    Source: Journal of Biomechanical Engineering:;1984:;volume( 106 ):;issue: 004::page 384
    Author:
    K. R. Diller
    ,
    D. A. Bradley
    DOI: 10.1115/1.3138510
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: The water permeability (Lp ) of human granulocytes was measured for individual isolated cells with a novel, microscopic stopped-flow mixing system. Changes in volume were monitored as a cell was introduced suddently into an osmotically active solution. Permeability values were determined as a function of solution osmolality from the volume versus time curves for mixing into both hypotonic and hypertonic solutions within the range of 145 to 833 mOsm. The calculated reference permeability at 25°C was 1.15 μm/atm·min with an osmotic coefficient of 0.46 Osm/kg.
    keyword(s): Flow (Dynamics) , Permeability AND Water ,
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      Measurement of the Water Permeability of Single Human Granulocytes on a Microscopic Stopped-Flow Mixing System

    URI
    http://yetl.yabesh.ir/yetl1/handle/yetl/98139
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    • Journal of Biomechanical Engineering

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    contributor authorK. R. Diller
    contributor authorD. A. Bradley
    date accessioned2017-05-08T23:17:19Z
    date available2017-05-08T23:17:19Z
    date copyrightNovember, 1984
    date issued1984
    identifier issn0148-0731
    identifier otherJBENDY-25795#384_1.pdf
    identifier urihttp://yetl.yabesh.ir/yetl/handle/yetl/98139
    description abstractThe water permeability (Lp ) of human granulocytes was measured for individual isolated cells with a novel, microscopic stopped-flow mixing system. Changes in volume were monitored as a cell was introduced suddently into an osmotically active solution. Permeability values were determined as a function of solution osmolality from the volume versus time curves for mixing into both hypotonic and hypertonic solutions within the range of 145 to 833 mOsm. The calculated reference permeability at 25°C was 1.15 μm/atm·min with an osmotic coefficient of 0.46 Osm/kg.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleMeasurement of the Water Permeability of Single Human Granulocytes on a Microscopic Stopped-Flow Mixing System
    typeJournal Paper
    journal volume106
    journal issue4
    journal titleJournal of Biomechanical Engineering
    identifier doi10.1115/1.3138510
    journal fristpage384
    journal lastpage393
    identifier eissn1528-8951
    keywordsFlow (Dynamics)
    keywordsPermeability AND Water
    treeJournal of Biomechanical Engineering:;1984:;volume( 106 ):;issue: 004
    contenttypeFulltext
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