| contributor author | E. G. Cravalho | |
| contributor author | C. E. Huggins | |
| contributor author | K. R. Diller | |
| contributor author | W. W. Watson | |
| date accessioned | 2017-05-08T23:10:41Z | |
| date available | 2017-05-08T23:10:41Z | |
| date copyright | February, 1981 | |
| date issued | 1981 | |
| identifier issn | 0148-0731 | |
| identifier other | JBENDY-25673#24_1.pdf | |
| identifier uri | http://yetl.yabesh.ir/yetl/handle/yetl/94309 | |
| description abstract | Human erythrocytes were collected in ACD anticoagulant, glycerolized with an equal volume of 8.6 M glycerol, 8 percent glucose, 1 percent fructose, and 0.3 percent Na2 EDTA solution, sealed in glass ampules, and frozen to −272.29°C (0.86°K) in liquid helium under high vacuum. Biological assays performed after thawing and three-stage dialysis to remove the glycerol showed less than 1 percent hemolysis. Intracellular potassium, 2,3 DPG, and ATP were within the normal range. | |
| publisher | The American Society of Mechanical Engineers (ASME) | |
| title | Blood Freezing to Nearly Absolute Zero Temperature: −272.29°C | |
| type | Journal Paper | |
| journal volume | 103 | |
| journal issue | 1 | |
| journal title | Journal of Biomechanical Engineering | |
| identifier doi | 10.1115/1.3138240 | |
| journal fristpage | 24 | |
| journal lastpage | 26 | |
| identifier eissn | 1528-8951 | |
| keywords | Temperature | |
| keywords | Freezing | |
| keywords | Glass | |
| keywords | Vacuum | |
| keywords | Absolute zero | |
| keywords | Assaying | |
| keywords | Thawing | |
| keywords | Blood | |
| keywords | Helium | |
| keywords | Potassium AND Erythrocytes | |
| tree | Journal of Biomechanical Engineering:;1981:;volume( 103 ):;issue: 001 | |
| contenttype | Fulltext | |
