| contributor author | Houchaimi, Sari | |
| contributor author | An, Hee Tae | |
| contributor author | Greyson, Marcette | |
| contributor author | Anzelone, Paul | |
| contributor author | Schertzer, Michael J. | |
| date accessioned | 2025-08-20T09:18:48Z | |
| date available | 2025-08-20T09:18:48Z | |
| date copyright | 3/28/2025 12:00:00 AM | |
| date issued | 2025 | |
| identifier issn | 2994-7316 | |
| identifier other | jmnm_013_02_021004.pdf | |
| identifier uri | http://yetl.yabesh.ir/yetl1/handle/yetl/4308073 | |
| description abstract | Digital microfluidics can be used to reliably automate repeated ligation steps to create accurate DNA products. This investigation demonstrates assembly of single-stranded and double-stranded DNA products on digital microfluidics devices. It also presents simple, low-cost methods to integrate on-chip incubation on a commonly used digital microfluidic platform. This work presents the ligation of single-stranded and double-stranded DNA products in droplets surrounded in oil and air with on-chip and off-chip incubations. Successful assembly of DNA products was determined by verifying that the size of on-chip DNA products was equal to benchtop controls. This suggests that digital microfluidic devices are a suitable platform for automated assembly of DNA products in a variety of biomedical applications. | |
| publisher | The American Society of Mechanical Engineers (ASME) | |
| title | Digital Microfluidics Automation of Oligonucleotide Assemblies | |
| type | Journal Paper | |
| journal volume | 13 | |
| journal issue | 2 | |
| journal title | Journal of Micro and Nano Science and Engineering | |
| identifier doi | 10.1115/1.4068003 | |
| journal fristpage | 21004-1 | |
| journal lastpage | 21004-10 | |
| page | 10 | |
| tree | Journal of Micro and Nano Science and Engineering:;2025:;volume( 013 ):;issue: 002 | |
| contenttype | Fulltext | |
