Flow Rate and Wall Shear Stress Characterization of a Biomimetic Aerosol Exposure SystemSource: Journal of Biomechanical Engineering:;2024:;volume( 146 ):;issue: 004::page 45001-1DOI: 10.1115/1.4064549Publisher: The American Society of Mechanical Engineers (ASME)
Abstract: Current in vitro emissions and exposure systems lack biomimicry, use unrealistic flow conditions, produce unrealistic dose, and provide inaccurate biomechanical cues to cell cultures, limiting ability to correlate in vitro outcomes with in vivo health effects. A biomimetic in vitro system capable of puffing aerosol and clean air inhalation may empower researchers to investigate complex questions related to lung injury and disease. A biomimetic aerosol exposure system (BAES), including an electronic cigarette adapter, oral cavity module (OCM), and bifurcated exposure chamber (BEC) was designed and manufactured. The fraction of aerosol deposited in transit to a filter pad or lost as volatiles was 0.116±0.021 in a traditional emissions setup versus 0.098 ± 0.015 with the adapter. The observed flowrate was within 5% of programed flowrate for puffing (25 mL/s), puff-associated respiration (450 mL/s), and tidal inhalation (350 mL/s). The maximum flowrate observed in the fabricated BAES was 450 mL/s, exceeding the lower target nominal wall shear stress of 0.025 Pa upstream of the bifurcation and fell below the target of 0.02 Pa downstream. This in vitro system addresses several gaps observed in commercially available systems and may be used to study many inhaled aerosols. The current work illustrates how in silico models may be used to correlate results of an in vitro study to in vivo conditions, rather than attempting to design an in vitro system that performs exactly as the human respiratory tract.
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contributor author | Emma Sarles, S. | |
contributor author | Hensel, Edward C. | |
contributor author | Terry, Janessa | |
contributor author | Nuss, Caleb | |
contributor author | Robinson, Risa J. | |
date accessioned | 2024-04-24T22:33:09Z | |
date available | 2024-04-24T22:33:09Z | |
date copyright | 2/9/2024 12:00:00 AM | |
date issued | 2024 | |
identifier issn | 0148-0731 | |
identifier other | bio_146_04_045001.pdf | |
identifier uri | http://yetl.yabesh.ir/yetl1/handle/yetl/4295431 | |
description abstract | Current in vitro emissions and exposure systems lack biomimicry, use unrealistic flow conditions, produce unrealistic dose, and provide inaccurate biomechanical cues to cell cultures, limiting ability to correlate in vitro outcomes with in vivo health effects. A biomimetic in vitro system capable of puffing aerosol and clean air inhalation may empower researchers to investigate complex questions related to lung injury and disease. A biomimetic aerosol exposure system (BAES), including an electronic cigarette adapter, oral cavity module (OCM), and bifurcated exposure chamber (BEC) was designed and manufactured. The fraction of aerosol deposited in transit to a filter pad or lost as volatiles was 0.116±0.021 in a traditional emissions setup versus 0.098 ± 0.015 with the adapter. The observed flowrate was within 5% of programed flowrate for puffing (25 mL/s), puff-associated respiration (450 mL/s), and tidal inhalation (350 mL/s). The maximum flowrate observed in the fabricated BAES was 450 mL/s, exceeding the lower target nominal wall shear stress of 0.025 Pa upstream of the bifurcation and fell below the target of 0.02 Pa downstream. This in vitro system addresses several gaps observed in commercially available systems and may be used to study many inhaled aerosols. The current work illustrates how in silico models may be used to correlate results of an in vitro study to in vivo conditions, rather than attempting to design an in vitro system that performs exactly as the human respiratory tract. | |
publisher | The American Society of Mechanical Engineers (ASME) | |
title | Flow Rate and Wall Shear Stress Characterization of a Biomimetic Aerosol Exposure System | |
type | Journal Paper | |
journal volume | 146 | |
journal issue | 4 | |
journal title | Journal of Biomechanical Engineering | |
identifier doi | 10.1115/1.4064549 | |
journal fristpage | 45001-1 | |
journal lastpage | 45001-11 | |
page | 11 | |
tree | Journal of Biomechanical Engineering:;2024:;volume( 146 ):;issue: 004 | |
contenttype | Fulltext |