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    Bioprinting Using Algae: Effects of Extrusion Pressure and Needle Diameter on Cell Quantity in Printed Samples

    Source: Journal of Manufacturing Science and Engineering:;2020:;volume( 143 ):;issue: 001::page 014501-1
    Author:
    Thakare, Ketan
    ,
    Jerpseth, Laura
    ,
    Qin, Hongmin
    ,
    Pei, Zhijian
    DOI: 10.1115/1.4048853
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: Bioprinting is the fabrication of structures based on layer-by-layer deposition of biomaterials. Applications of bioprinting using plant or algae cells include the production of metabolites for use in pharmaceutical, cosmetic, and food industries. Reported studies regarding effects of extrusion pressure and needle diameter on cell viability in bioprinting have used animal cells. There are no reports regarding effects of extrusion pressure and needle diameter on cell viability using plant or algae cells. This paper fills this knowledge gap by reporting an experimental investigation on effects of extrusion pressure and needle diameter on cell quantity (an indicator of cell viability) in extrusion-based bioprinting of hydrogel-based bioink containing Chlamydomonas reinhardtii algae cells. Extrusion pressure levels used in this study were 3, 5, and 7 bar, and needle diameter levels were 200, 250, and 400 µm. Algae cell quantity in printed samples was measured on the third day and sixth day post bioprinting. Results show that, when extrusion pressure increases or needle diameter decreases, algae cell quantity in printed samples decreases.
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      Bioprinting Using Algae: Effects of Extrusion Pressure and Needle Diameter on Cell Quantity in Printed Samples

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    http://yetl.yabesh.ir/yetl1/handle/yetl/4276124
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    contributor authorThakare, Ketan
    contributor authorJerpseth, Laura
    contributor authorQin, Hongmin
    contributor authorPei, Zhijian
    date accessioned2022-02-05T21:40:47Z
    date available2022-02-05T21:40:47Z
    date copyright12/3/2020 12:00:00 AM
    date issued2020
    identifier issn1087-1357
    identifier othermanu_143_1_014501.pdf
    identifier urihttp://yetl.yabesh.ir/yetl1/handle/yetl/4276124
    description abstractBioprinting is the fabrication of structures based on layer-by-layer deposition of biomaterials. Applications of bioprinting using plant or algae cells include the production of metabolites for use in pharmaceutical, cosmetic, and food industries. Reported studies regarding effects of extrusion pressure and needle diameter on cell viability in bioprinting have used animal cells. There are no reports regarding effects of extrusion pressure and needle diameter on cell viability using plant or algae cells. This paper fills this knowledge gap by reporting an experimental investigation on effects of extrusion pressure and needle diameter on cell quantity (an indicator of cell viability) in extrusion-based bioprinting of hydrogel-based bioink containing Chlamydomonas reinhardtii algae cells. Extrusion pressure levels used in this study were 3, 5, and 7 bar, and needle diameter levels were 200, 250, and 400 µm. Algae cell quantity in printed samples was measured on the third day and sixth day post bioprinting. Results show that, when extrusion pressure increases or needle diameter decreases, algae cell quantity in printed samples decreases.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleBioprinting Using Algae: Effects of Extrusion Pressure and Needle Diameter on Cell Quantity in Printed Samples
    typeJournal Paper
    journal volume143
    journal issue1
    journal titleJournal of Manufacturing Science and Engineering
    identifier doi10.1115/1.4048853
    journal fristpage014501-1
    journal lastpage014501-5
    page5
    treeJournal of Manufacturing Science and Engineering:;2020:;volume( 143 ):;issue: 001
    contenttypeFulltext
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