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    An Ultrafast Vitrification Method for Cell Cryopreservation

    Source: Journal of Heat Transfer:;2018:;volume( 140 ):;issue: 001::page 12001
    Author:
    Su, Fengmin
    ,
    Zhao, Nannan
    ,
    Deng, Yangbo
    ,
    Ma, Hongbin
    DOI: 10.1115/1.4037327
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: Ultrafast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultrafast cooling rate. Experimental results show that the average cooling rate of dimethylsulfoxide (DMSO) cryoprotective solution reaches 150,000 °C/min in a temperature range from 10 °C to −180 °C. The ultrafast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.
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      An Ultrafast Vitrification Method for Cell Cryopreservation

    URI
    http://yetl.yabesh.ir/yetl1/handle/yetl/4251849
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    contributor authorSu, Fengmin
    contributor authorZhao, Nannan
    contributor authorDeng, Yangbo
    contributor authorMa, Hongbin
    date accessioned2019-02-28T11:01:33Z
    date available2019-02-28T11:01:33Z
    date copyright8/16/2017 12:00:00 AM
    date issued2018
    identifier issn0022-1481
    identifier otherht_140_01_012001.pdf
    identifier urihttp://yetl.yabesh.ir/yetl1/handle/yetl/4251849
    description abstractUltrafast cooling is the key to successful cell vitrification cryopreservation of lower concentration cryoprotective solution. This research develops a cell cryopreservation methodology which utilizes thin film evaporation and achieves vitrification of relatively low concentration cryoprotectant with an ultrafast cooling rate. Experimental results show that the average cooling rate of dimethylsulfoxide (DMSO) cryoprotective solution reaches 150,000 °C/min in a temperature range from 10 °C to −180 °C. The ultrafast cooling rate can remarkably improve the vitrification tendencies of the cryoprotective solution. This methodology opens the possibility for more successful cell vitrification cryopreservation.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleAn Ultrafast Vitrification Method for Cell Cryopreservation
    typeJournal Paper
    journal volume140
    journal issue1
    journal titleJournal of Heat Transfer
    identifier doi10.1115/1.4037327
    journal fristpage12001
    journal lastpage012001-4
    treeJournal of Heat Transfer:;2018:;volume( 140 ):;issue: 001
    contenttypeFulltext
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    DSpace software copyright © 2002-2015  DuraSpace
    نرم افزار کتابخانه دیجیتال "دی اسپیس" فارسی شده توسط یابش برای کتابخانه های ایرانی | تماس با یابش
    yabeshDSpacePersian