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    Interaction of <i>Microcystis</i> and Phix174 in the Aquatic Environment

    Source: Journal of Environmental Engineering:;2017:;Volume ( 143 ):;issue: 006
    Author:
    Chen-Xi Sun
    ,
    Genevieve G. R. V. Vergara
    ,
    Karina Yew-Hoong Gin
    DOI: 10.1061/(ASCE)EE.1943-7870.0001208
    Publisher: American Society of Civil Engineers
    Abstract: This study aimed to explore the potential impact of algal blooms on the survival of viruses in aquatic environments by using Microcystis and somatic coliphage phiX174 as a model algae and model virus, respectively. The phiX174 concentration was determined by double-agar-layer plaque assay (DAL), and the Microcystis density was determined by optical density (OD) measurement at 678 nm. Individual samples were measured before and after filtration through a membrane with pore size of 0.22 μm to account for the differences between unassociated viruses suspended in the water column and the total viable viruses in the sample. The results showed that no significant inactivation or adsorption of phiX174 was caused by Microcystis either in dark condition or under light. However, algae could act as a source of natural organic matter (NOM) and affect phiX174 inactivation. The presence of algae could also reduce phiX174 inactivation because of light attenuation. Therefore, the presence of algae could potentially prolong the survival of virus in aquatic environment.
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      Interaction of <i>Microcystis</i> and Phix174 in the Aquatic Environment

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    http://yetl.yabesh.ir/yetl1/handle/yetl/4240832
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    contributor authorChen-Xi Sun
    contributor authorGenevieve G. R. V. Vergara
    contributor authorKarina Yew-Hoong Gin
    date accessioned2017-12-16T09:16:33Z
    date available2017-12-16T09:16:33Z
    date issued2017
    identifier other%28ASCE%29EE.1943-7870.0001208.pdf
    identifier urihttp://138.201.223.254:8080/yetl1/handle/yetl/4240832
    description abstractThis study aimed to explore the potential impact of algal blooms on the survival of viruses in aquatic environments by using Microcystis and somatic coliphage phiX174 as a model algae and model virus, respectively. The phiX174 concentration was determined by double-agar-layer plaque assay (DAL), and the Microcystis density was determined by optical density (OD) measurement at 678 nm. Individual samples were measured before and after filtration through a membrane with pore size of 0.22 μm to account for the differences between unassociated viruses suspended in the water column and the total viable viruses in the sample. The results showed that no significant inactivation or adsorption of phiX174 was caused by Microcystis either in dark condition or under light. However, algae could act as a source of natural organic matter (NOM) and affect phiX174 inactivation. The presence of algae could also reduce phiX174 inactivation because of light attenuation. Therefore, the presence of algae could potentially prolong the survival of virus in aquatic environment.
    publisherAmerican Society of Civil Engineers
    titleInteraction of Microcystis and Phix174 in the Aquatic Environment
    typeJournal Paper
    journal volume143
    journal issue6
    journal titleJournal of Environmental Engineering
    identifier doi10.1061/(ASCE)EE.1943-7870.0001208
    treeJournal of Environmental Engineering:;2017:;Volume ( 143 ):;issue: 006
    contenttypeFulltext
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