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contributor authorChen-Xi Sun
contributor authorGenevieve G. R. V. Vergara
contributor authorKarina Yew-Hoong Gin
date accessioned2017-12-16T09:16:33Z
date available2017-12-16T09:16:33Z
date issued2017
identifier other%28ASCE%29EE.1943-7870.0001208.pdf
identifier urihttp://138.201.223.254:8080/yetl1/handle/yetl/4240832
description abstractThis study aimed to explore the potential impact of algal blooms on the survival of viruses in aquatic environments by using Microcystis and somatic coliphage phiX174 as a model algae and model virus, respectively. The phiX174 concentration was determined by double-agar-layer plaque assay (DAL), and the Microcystis density was determined by optical density (OD) measurement at 678 nm. Individual samples were measured before and after filtration through a membrane with pore size of 0.22 μm to account for the differences between unassociated viruses suspended in the water column and the total viable viruses in the sample. The results showed that no significant inactivation or adsorption of phiX174 was caused by Microcystis either in dark condition or under light. However, algae could act as a source of natural organic matter (NOM) and affect phiX174 inactivation. The presence of algae could also reduce phiX174 inactivation because of light attenuation. Therefore, the presence of algae could potentially prolong the survival of virus in aquatic environment.
publisherAmerican Society of Civil Engineers
titleInteraction of Microcystis and Phix174 in the Aquatic Environment
typeJournal Paper
journal volume143
journal issue6
journal titleJournal of Environmental Engineering
identifier doi10.1061/(ASCE)EE.1943-7870.0001208
treeJournal of Environmental Engineering:;2017:;Volume ( 143 ):;issue: 006
contenttypeFulltext


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