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    Cyclic Mechanical Loading Enhances Transport of Antibodies Into Articular Cartilage

    Source: Journal of Biomechanical Engineering:;2017:;volume( 139 ):;issue: 001::page 11012
    Author:
    DiDomenico, Chris D.
    ,
    Xiang Wang, Zhen
    ,
    Bonassar, Lawrence J.
    DOI: 10.1115/1.4035265
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: The goal of this study was to characterize antibody penetration through cartilage tissue under mechanical loading. Mechanical stimulation aids in the penetration of some proteins, but this effect has not characterized molecules such as antibodies (>100 kDa), which may hold some clinical value for treating osteoarthritis (OA). For each experiment, fresh articular cartilage plugs were obtained and exposed to fluorescently labeled antibodies while under cyclic mechanical load in unconfined compression for several hours. Penetration of these antibodies was quantified using confocal microscopy, and finite element (FE) simulations were conducted to predict fluid flow patterns within loaded samples. Transport enhancement followed a linear trend with strain amplitude (0.25–5%) and a nonlinear trend with frequency (0.25–2.60 Hz), with maximum enhancement found to be at 5% cyclic strain and 1 Hz, respectively. Regions of highest enhancement of transport within the tissue were associated with the regions of highest interstitial fluid velocity, as predicted from finite-element simulations. Overall, cyclic compression-enhanced antibody transport by twofold to threefold. To our knowledge, this is the first study to test how mechanical stimulation affects the diffusion of antibodies in cartilage and suggest further study into other important factors regarding macromolecular transport.
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      Cyclic Mechanical Loading Enhances Transport of Antibodies Into Articular Cartilage

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    http://yetl.yabesh.ir/yetl1/handle/yetl/4235130
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    • Journal of Biomechanical Engineering

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    contributor authorDiDomenico, Chris D.
    contributor authorXiang Wang, Zhen
    contributor authorBonassar, Lawrence J.
    date accessioned2017-11-25T07:18:20Z
    date available2017-11-25T07:18:20Z
    date copyright2016/30/11
    date issued2017
    identifier issn0148-0731
    identifier otherbio_139_01_011012.pdf
    identifier urihttp://138.201.223.254:8080/yetl1/handle/yetl/4235130
    description abstractThe goal of this study was to characterize antibody penetration through cartilage tissue under mechanical loading. Mechanical stimulation aids in the penetration of some proteins, but this effect has not characterized molecules such as antibodies (>100 kDa), which may hold some clinical value for treating osteoarthritis (OA). For each experiment, fresh articular cartilage plugs were obtained and exposed to fluorescently labeled antibodies while under cyclic mechanical load in unconfined compression for several hours. Penetration of these antibodies was quantified using confocal microscopy, and finite element (FE) simulations were conducted to predict fluid flow patterns within loaded samples. Transport enhancement followed a linear trend with strain amplitude (0.25–5%) and a nonlinear trend with frequency (0.25–2.60 Hz), with maximum enhancement found to be at 5% cyclic strain and 1 Hz, respectively. Regions of highest enhancement of transport within the tissue were associated with the regions of highest interstitial fluid velocity, as predicted from finite-element simulations. Overall, cyclic compression-enhanced antibody transport by twofold to threefold. To our knowledge, this is the first study to test how mechanical stimulation affects the diffusion of antibodies in cartilage and suggest further study into other important factors regarding macromolecular transport.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleCyclic Mechanical Loading Enhances Transport of Antibodies Into Articular Cartilage
    typeJournal Paper
    journal volume139
    journal issue1
    journal titleJournal of Biomechanical Engineering
    identifier doi10.1115/1.4035265
    journal fristpage11012
    journal lastpage011012-7
    treeJournal of Biomechanical Engineering:;2017:;volume( 139 ):;issue: 001
    contenttypeFulltext
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