YaBeSH Engineering and Technology Library

    • Journals
    • PaperQuest
    • YSE Standards
    • YaBeSH
    • Login
    View Item 
    •   YE&T Library
    • ASME
    • Journal of Nanotechnology in Engineering and Medicine
    • View Item
    •   YE&T Library
    • ASME
    • Journal of Nanotechnology in Engineering and Medicine
    • View Item
    • All Fields
    • Source Title
    • Year
    • Publisher
    • Title
    • Subject
    • Author
    • DOI
    • ISBN
    Advanced Search
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Archive

    A Novel Suspended Hydrogel Membrane Platform for Cell Culture

    Source: Journal of Nanotechnology in Engineering and Medicine:;2015:;volume( 006 ):;issue: 002::page 21002
    Author:
    Chen, Yong X.
    ,
    Yang, Shihao
    ,
    Yan, Jiahan
    ,
    Hsieh, Ming
    ,
    Weng, Lingyan
    ,
    Ouderkirk, Jessica L.
    ,
    Krendel, Mira
    ,
    Soman, Pranav
    DOI: 10.1115/1.4031467
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: Current cellculture is largely performed on synthetic twodimensional (2D) petri dishes or permeable supports such as Boyden chambers, mostly because of their ease of use and established protocols. It is generally accepted that modern cell biology research requires new physiologically relevant threedimensional (3D) cell culture platform to mimic in vivo cell responses. To that end, we report the design and development of a suspended hydrogel membrane (ShyM) platform using gelatin methacrylate (GelMA) hydrogel. ShyM thickness (0.25–1 mm) and mechanical properties (10–70 kPa) can be varied by controlling the size of the supporting grid and concentration of GelMA prepolymer, respectively. GelMA ShyMs, with dual media exposure, were found to be compatible with both the cellseeding and the cellencapsulation approach as tested using murine 10T1/2 cells and demonstrated higher cellular spreading and proliferation as compared to flat GelMA unsuspended control. The utility of ShyM was also demonstrated using a casestudy of invasion of cancer cells. ShyMs, similar to Boyden chambers, are compatible with standard wellplates designs and can be printed using commonly available 3D printers. In the future, ShyM can be potentially extended to variety of photosensitive hydrogels and cell types, to develop new in vitro assays to investigate complex cell–cell and cell–extracellular matrix (ECM) interactions.
    • Download: (2.713Mb)
    • Show Full MetaData Hide Full MetaData
    • Get RIS
    • Item Order
    • Go To Publisher
    • Price: 5000 Rial
    • Statistics

      A Novel Suspended Hydrogel Membrane Platform for Cell Culture

    URI
    http://yetl.yabesh.ir/yetl1/handle/yetl/159262
    Collections
    • Journal of Nanotechnology in Engineering and Medicine

    Show full item record

    contributor authorChen, Yong X.
    contributor authorYang, Shihao
    contributor authorYan, Jiahan
    contributor authorHsieh, Ming
    contributor authorWeng, Lingyan
    contributor authorOuderkirk, Jessica L.
    contributor authorKrendel, Mira
    contributor authorSoman, Pranav
    date accessioned2017-05-09T01:22:12Z
    date available2017-05-09T01:22:12Z
    date issued2015
    identifier issn1949-2944
    identifier othernano_006_02_021002.pdf
    identifier urihttp://yetl.yabesh.ir/yetl/handle/yetl/159262
    description abstractCurrent cellculture is largely performed on synthetic twodimensional (2D) petri dishes or permeable supports such as Boyden chambers, mostly because of their ease of use and established protocols. It is generally accepted that modern cell biology research requires new physiologically relevant threedimensional (3D) cell culture platform to mimic in vivo cell responses. To that end, we report the design and development of a suspended hydrogel membrane (ShyM) platform using gelatin methacrylate (GelMA) hydrogel. ShyM thickness (0.25–1 mm) and mechanical properties (10–70 kPa) can be varied by controlling the size of the supporting grid and concentration of GelMA prepolymer, respectively. GelMA ShyMs, with dual media exposure, were found to be compatible with both the cellseeding and the cellencapsulation approach as tested using murine 10T1/2 cells and demonstrated higher cellular spreading and proliferation as compared to flat GelMA unsuspended control. The utility of ShyM was also demonstrated using a casestudy of invasion of cancer cells. ShyMs, similar to Boyden chambers, are compatible with standard wellplates designs and can be printed using commonly available 3D printers. In the future, ShyM can be potentially extended to variety of photosensitive hydrogels and cell types, to develop new in vitro assays to investigate complex cell–cell and cell–extracellular matrix (ECM) interactions.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleA Novel Suspended Hydrogel Membrane Platform for Cell Culture
    typeJournal Paper
    journal volume6
    journal issue2
    journal titleJournal of Nanotechnology in Engineering and Medicine
    identifier doi10.1115/1.4031467
    journal fristpage21002
    journal lastpage21002
    identifier eissn1949-2952
    treeJournal of Nanotechnology in Engineering and Medicine:;2015:;volume( 006 ):;issue: 002
    contenttypeFulltext
    DSpace software copyright © 2002-2015  DuraSpace
    نرم افزار کتابخانه دیجیتال "دی اسپیس" فارسی شده توسط یابش برای کتابخانه های ایرانی | تماس با یابش
    yabeshDSpacePersian
     
    DSpace software copyright © 2002-2015  DuraSpace
    نرم افزار کتابخانه دیجیتال "دی اسپیس" فارسی شده توسط یابش برای کتابخانه های ایرانی | تماس با یابش
    yabeshDSpacePersian