The Effects of Elastic Fiber Protein Insufficiency and Treatment on the Modulus of Arterial Smooth Muscle CellsSource: Journal of Biomechanical Engineering:;2014:;volume( 136 ):;issue: 002::page 21030Author:Gabriela Espinosa, M.
,
Gardner, William S.
,
Bennett, Lisa
,
Sather, Bradley A.
,
Yanagisawa, Hiromi
,
Wagenseil, Jessica E.
DOI: 10.1115/1.4026203Publisher: The American Society of Mechanical Engineers (ASME)
Abstract: Elastic fibers are critical for the mechanical function of the large arteries. Mechanical effects of elastic fiber protein deficiency have been investigated in whole arteries, but not in isolated smooth muscle cells (SMCs). The elastic moduli of SMCs from elastin (Eln/) and fibulin4 (Fbln4/) knockout mice were measured using atomic force microscopy. Compared to control SMCs, the modulus of Eln/SMCs is reduced by 40%, but is unchanged in Fbln4/SMCs. The Eln/SMC modulus is rescued by soluble or خ± elastin treatment. Altered gene expression, specifically of calponin, suggests that SMC phenotypic modulation may be responsible for the modulus changes.
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| contributor author | Gabriela Espinosa, M. | |
| contributor author | Gardner, William S. | |
| contributor author | Bennett, Lisa | |
| contributor author | Sather, Bradley A. | |
| contributor author | Yanagisawa, Hiromi | |
| contributor author | Wagenseil, Jessica E. | |
| date accessioned | 2017-05-09T01:05:19Z | |
| date available | 2017-05-09T01:05:19Z | |
| date issued | 2014 | |
| identifier issn | 0148-0731 | |
| identifier other | bio_136_02_021030.pdf | |
| identifier uri | http://yetl.yabesh.ir/yetl/handle/yetl/153971 | |
| description abstract | Elastic fibers are critical for the mechanical function of the large arteries. Mechanical effects of elastic fiber protein deficiency have been investigated in whole arteries, but not in isolated smooth muscle cells (SMCs). The elastic moduli of SMCs from elastin (Eln/) and fibulin4 (Fbln4/) knockout mice were measured using atomic force microscopy. Compared to control SMCs, the modulus of Eln/SMCs is reduced by 40%, but is unchanged in Fbln4/SMCs. The Eln/SMC modulus is rescued by soluble or خ± elastin treatment. Altered gene expression, specifically of calponin, suggests that SMC phenotypic modulation may be responsible for the modulus changes. | |
| publisher | The American Society of Mechanical Engineers (ASME) | |
| title | The Effects of Elastic Fiber Protein Insufficiency and Treatment on the Modulus of Arterial Smooth Muscle Cells | |
| type | Journal Paper | |
| journal volume | 136 | |
| journal issue | 2 | |
| journal title | Journal of Biomechanical Engineering | |
| identifier doi | 10.1115/1.4026203 | |
| journal fristpage | 21030 | |
| journal lastpage | 21030 | |
| identifier eissn | 1528-8951 | |
| tree | Journal of Biomechanical Engineering:;2014:;volume( 136 ):;issue: 002 | |
| contenttype | Fulltext |