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    A Novel Multiwell Device to Study Vascular Smooth Muscle Cell Responses Under Cyclic Strain

    Source: Journal of Nanotechnology in Engineering and Medicine:;2011:;volume( 002 ):;issue: 002::page 21007
    Author:
    Uday Tata
    ,
    Hao Xu
    ,
    Smitha M. N. Rao
    ,
    Cheng-Jen Chuong
    ,
    J.-C. Chiao
    ,
    Kytai T. Nguyen
    DOI: 10.1115/1.4003928
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: Vascular smooth muscle cells (VSMCs) are constantly exposed to cyclic stretch in the body, which makes it beneficial to study the effects of cyclic stretch on VSMCs. In this study, we developed a poly(dimethyl siloxane) (PDMS) compact six-well device that can be used to study the combined effect of cyclic strain and various growth factors on cultured VSMCs. Cell adhesion, alignment, and proliferation under 10% or 20% cyclic strain at 1 Hz were studied using this surface-enhanced PDMS device. The combined effects of cyclic strain with either transforming growth factor-β, vascular endothelial growth factor, fibroblast growth factor, or epidermal growth factor on VSMC proliferation was also examined. Results showed that VSMCs adhered well on the surface-enhanced multiwell device and they aligned perpendicularly to the direction of the cyclic strain. Cell proliferation was inhibited by 10% cyclic strain at 1 Hz compared with static control. The mitogenic effects of the growth factor were less potent under either 10% or 20% cyclic strain. With simple modification to accommodate more wells, this device could potentially be a useful tool for more economical, high throughput screening application.
    keyword(s): Plasma desorption mass spectrometry , Muscle , Wells , Siloxanes , Fibroblasts , Vacuum AND Cultured cells ,
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      A Novel Multiwell Device to Study Vascular Smooth Muscle Cell Responses Under Cyclic Strain

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    http://yetl.yabesh.ir/yetl1/handle/yetl/147317
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    contributor authorUday Tata
    contributor authorHao Xu
    contributor authorSmitha M. N. Rao
    contributor authorCheng-Jen Chuong
    contributor authorJ.-C. Chiao
    contributor authorKytai T. Nguyen
    date accessioned2017-05-09T00:46:21Z
    date available2017-05-09T00:46:21Z
    date copyrightMay, 2011
    date issued2011
    identifier issn1949-2944
    identifier otherJNEMAA-28057#021007_1.pdf
    identifier urihttp://yetl.yabesh.ir/yetl/handle/yetl/147317
    description abstractVascular smooth muscle cells (VSMCs) are constantly exposed to cyclic stretch in the body, which makes it beneficial to study the effects of cyclic stretch on VSMCs. In this study, we developed a poly(dimethyl siloxane) (PDMS) compact six-well device that can be used to study the combined effect of cyclic strain and various growth factors on cultured VSMCs. Cell adhesion, alignment, and proliferation under 10% or 20% cyclic strain at 1 Hz were studied using this surface-enhanced PDMS device. The combined effects of cyclic strain with either transforming growth factor-β, vascular endothelial growth factor, fibroblast growth factor, or epidermal growth factor on VSMC proliferation was also examined. Results showed that VSMCs adhered well on the surface-enhanced multiwell device and they aligned perpendicularly to the direction of the cyclic strain. Cell proliferation was inhibited by 10% cyclic strain at 1 Hz compared with static control. The mitogenic effects of the growth factor were less potent under either 10% or 20% cyclic strain. With simple modification to accommodate more wells, this device could potentially be a useful tool for more economical, high throughput screening application.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleA Novel Multiwell Device to Study Vascular Smooth Muscle Cell Responses Under Cyclic Strain
    typeJournal Paper
    journal volume2
    journal issue2
    journal titleJournal of Nanotechnology in Engineering and Medicine
    identifier doi10.1115/1.4003928
    journal fristpage21007
    identifier eissn1949-2952
    keywordsPlasma desorption mass spectrometry
    keywordsMuscle
    keywordsWells
    keywordsSiloxanes
    keywordsFibroblasts
    keywordsVacuum AND Cultured cells
    treeJournal of Nanotechnology in Engineering and Medicine:;2011:;volume( 002 ):;issue: 002
    contenttypeFulltext
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    DSpace software copyright © 2002-2015  DuraSpace
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