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    Theoretical Study on Temperature Dependence of Cellular Uptake of QDs Nanoparticles

    Source: Journal of Biomechanical Engineering:;2011:;volume( 133 ):;issue: 012::page 124502
    Author:
    Aili Zhang
    ,
    Lisa X. Xu
    ,
    Yingxue Guan
    DOI: 10.1115/1.4005481
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: Cellular uptake kinetics of nanoparticles is one of the key issues determining the design and application of the particles. Models describing nanoparticles intrusion into the cell mostly take the endocytosis process into consideration, and the influences of electrical charges, sizes, concentrations of the particles have been investigated. In this paper, the temperature effect on the cellular uptake of Quantum Dots (QDs) is studied experimentally. QDs are incubated with the SPCA-1 human lung tumor cells, and the nanoparticles on the cell membrane and inside the cell are quantified according to the fluorescence intensities recorded. It is found that the amounts of nanoparticles attached onto the cell membrane and inside the cell both increase with temperature. Based on the experimental results, a model is proposed to describe the cellular uptake dynamic process of nanoparticles. The process consists of two steps: nanoparticles adsorption onto the cell membrane and the internalization. The dynamic parameters are obtained through curve fitting. The simulated results show that the internalization process can be categorized into different phases. The temperature dependent internalization rate constant is very small when below 14 °C. It increases distinctly when temperature rises from 14 °C to 22 °C, but there is no evident increase as temperature further increases above 22 °C. Results show that by incorporating a temperature-independent internalization factor, the model predictions well fit the experimental results.
    keyword(s): Temperature , Nanoparticles , Fluorescence AND Membranes ,
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      Theoretical Study on Temperature Dependence of Cellular Uptake of QDs Nanoparticles

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    http://yetl.yabesh.ir/yetl1/handle/yetl/145344
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    contributor authorAili Zhang
    contributor authorLisa X. Xu
    contributor authorYingxue Guan
    date accessioned2017-05-09T00:42:17Z
    date available2017-05-09T00:42:17Z
    date copyrightDecember, 2011
    date issued2011
    identifier issn0148-0731
    identifier otherJBENDY-27235#124502_1.pdf
    identifier urihttp://yetl.yabesh.ir/yetl/handle/yetl/145344
    description abstractCellular uptake kinetics of nanoparticles is one of the key issues determining the design and application of the particles. Models describing nanoparticles intrusion into the cell mostly take the endocytosis process into consideration, and the influences of electrical charges, sizes, concentrations of the particles have been investigated. In this paper, the temperature effect on the cellular uptake of Quantum Dots (QDs) is studied experimentally. QDs are incubated with the SPCA-1 human lung tumor cells, and the nanoparticles on the cell membrane and inside the cell are quantified according to the fluorescence intensities recorded. It is found that the amounts of nanoparticles attached onto the cell membrane and inside the cell both increase with temperature. Based on the experimental results, a model is proposed to describe the cellular uptake dynamic process of nanoparticles. The process consists of two steps: nanoparticles adsorption onto the cell membrane and the internalization. The dynamic parameters are obtained through curve fitting. The simulated results show that the internalization process can be categorized into different phases. The temperature dependent internalization rate constant is very small when below 14 °C. It increases distinctly when temperature rises from 14 °C to 22 °C, but there is no evident increase as temperature further increases above 22 °C. Results show that by incorporating a temperature-independent internalization factor, the model predictions well fit the experimental results.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleTheoretical Study on Temperature Dependence of Cellular Uptake of QDs Nanoparticles
    typeJournal Paper
    journal volume133
    journal issue12
    journal titleJournal of Biomechanical Engineering
    identifier doi10.1115/1.4005481
    journal fristpage124502
    identifier eissn1528-8951
    keywordsTemperature
    keywordsNanoparticles
    keywordsFluorescence AND Membranes
    treeJournal of Biomechanical Engineering:;2011:;volume( 133 ):;issue: 012
    contenttypeFulltext
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