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    Demonstration of Cancer Cell Migration Using a Novel Microfluidic Device

    Source: Journal of Nanotechnology in Engineering and Medicine:;2010:;volume( 001 ):;issue: 002::page 21003
    Author:
    Smitha M. N. Rao
    ,
    Victor K. Lin
    ,
    Uday Tata
    ,
    Ganesh V. Raj
    ,
    Kytai Nguyen
    ,
    J.-C. Chiao
    ,
    Jer-Tsong Hsieh
    DOI: 10.1115/1.4001280
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: Migration of cancer cells from the primary organ site via the bloodstream to distant sites is critical to the development of malignant metastasis and is in part determined by soluble host factors in the serum. Conventional Boyden chamber assays to evaluate cell motility require high volumes of reagents and are impractical for high-throughput analysis. We have designed and evaluated a poly-dimethylsiloxane (PDMS) microfluidic device in order to systematically study cancer cell migration. Photolithography and soft lithography processes were used to fabricate the PDMS devices from a negative photoresist (SU-8) mold. The device provides two separate identical chambers that are interconnected by an array of identical narrow channels, 10 μm high, 25 μm wide, and 1000 μm long. One chamber is seeded with cancer cells whose migration characteristics are to be evaluated, while the other chamber contains media with chemoattractants toward which the cancer cells migrate. In this microfluidic chamber model, the migration of cancer cells within and across the microfluidic channels over a prescribed time was quantified using time-lapse photographs. The microfluidic chamber is a cost-effective platform that uses small volumes of reagents, can maintain stable chemokine gradients, allow real-time quantitative study of cancer cell migration, and provide information about cellular dynamics and biomechanical analysis. This work demonstrated the utility of the microfluidic device as a platform to study cancer cell migration as well as the potential applications in the identification of specific chemokine agents and development of drugs targeting cell migration.
    keyword(s): Channels (Hydraulic engineering) , Plasma desorption mass spectrometry , Microfluidics , Cancer AND Gradients ,
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      Demonstration of Cancer Cell Migration Using a Novel Microfluidic Device

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    http://yetl.yabesh.ir/yetl1/handle/yetl/144543
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    contributor authorSmitha M. N. Rao
    contributor authorVictor K. Lin
    contributor authorUday Tata
    contributor authorGanesh V. Raj
    contributor authorKytai Nguyen
    contributor authorJ.-C. Chiao
    contributor authorJer-Tsong Hsieh
    date accessioned2017-05-09T00:40:15Z
    date available2017-05-09T00:40:15Z
    date copyrightMay, 2010
    date issued2010
    identifier issn1949-2944
    identifier otherJNEMAA-28035#021003_1.pdf
    identifier urihttp://yetl.yabesh.ir/yetl/handle/yetl/144543
    description abstractMigration of cancer cells from the primary organ site via the bloodstream to distant sites is critical to the development of malignant metastasis and is in part determined by soluble host factors in the serum. Conventional Boyden chamber assays to evaluate cell motility require high volumes of reagents and are impractical for high-throughput analysis. We have designed and evaluated a poly-dimethylsiloxane (PDMS) microfluidic device in order to systematically study cancer cell migration. Photolithography and soft lithography processes were used to fabricate the PDMS devices from a negative photoresist (SU-8) mold. The device provides two separate identical chambers that are interconnected by an array of identical narrow channels, 10 μm high, 25 μm wide, and 1000 μm long. One chamber is seeded with cancer cells whose migration characteristics are to be evaluated, while the other chamber contains media with chemoattractants toward which the cancer cells migrate. In this microfluidic chamber model, the migration of cancer cells within and across the microfluidic channels over a prescribed time was quantified using time-lapse photographs. The microfluidic chamber is a cost-effective platform that uses small volumes of reagents, can maintain stable chemokine gradients, allow real-time quantitative study of cancer cell migration, and provide information about cellular dynamics and biomechanical analysis. This work demonstrated the utility of the microfluidic device as a platform to study cancer cell migration as well as the potential applications in the identification of specific chemokine agents and development of drugs targeting cell migration.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleDemonstration of Cancer Cell Migration Using a Novel Microfluidic Device
    typeJournal Paper
    journal volume1
    journal issue2
    journal titleJournal of Nanotechnology in Engineering and Medicine
    identifier doi10.1115/1.4001280
    journal fristpage21003
    identifier eissn1949-2952
    keywordsChannels (Hydraulic engineering)
    keywordsPlasma desorption mass spectrometry
    keywordsMicrofluidics
    keywordsCancer AND Gradients
    treeJournal of Nanotechnology in Engineering and Medicine:;2010:;volume( 001 ):;issue: 002
    contenttypeFulltext
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    DSpace software copyright © 2002-2015  DuraSpace
    نرم افزار کتابخانه دیجیتال "دی اسپیس" فارسی شده توسط یابش برای کتابخانه های ایرانی | تماس با یابش
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