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    In Vivo Model for Evaluating the Effects of Mechanical Stimulation on Tissue-Engineered Bone Repair

    Source: Journal of Biomechanical Engineering:;2009:;volume( 131 ):;issue: 008::page 84502
    Author:
    Joel D. Boerckel
    ,
    Yash M. Kolambkar
    ,
    Angela S. P. Lin
    ,
    Robert E. Guldberg
    ,
    Kenneth M. Dupont
    DOI: 10.1115/1.3148472
    Publisher: The American Society of Mechanical Engineers (ASME)
    Abstract: It has long been known that the bone adapts according to the local mechanical environment. To date, however, a model for studying the effects of functional mechanical loading on tissue-engineered bone repair in vivo has not yet been established. We have developed a rat femoral defect model, in which ambulatory loads are transduced through the implanted tissue-engineered construct to elucidate the role of the mechanical environment in functional restoration of a large bone defect. This model uses compliant fixation plates with integrated elastomeric segments, which allow transduction of ambulatory loads. Multiaxially and uniaxially compliant plates were characterized by mechanical testing and evaluated using in vivo pilot studies. In the first study, experimental limbs were implanted with multiaxial plates, which have a low stiffness in multiple loading modes. In the second study, experimental limbs were stabilized by a uniaxial plate, which allowed only axial deformation of the defect. X-ray scans and mechanical testing revealed that the multiaxial plates were insufficient to stabilize the defect and prevent fracture under ambulatory loads as a result of low flexural and torsional stiffness. The uniaxial plates, however, maintained integrity of the defect when implanted over a 12 week period. Postmortem microCT scans revealed a 19% increase in bone volume in the axially loaded limb compared with the contralateral standard control, and postmortem mechanical testing indicated that torsional strength and stiffness were increased 25.6- and 3.9-fold, respectively, compared with the control. Finite element modeling revealed high strain gradients in the soft tissue adjacent to the newly formed bone within the implanted construct. This study introduces an in vivo model for studying the effects of physiological mechanical loading on tissue-engineered bone repair. Preliminary results using this new in vivo model with the uniaxially compliant plate showed positive effects of load-bearing on functional defect repair.
    keyword(s): Maintenance , Stress , Biological tissues , Bone , Finite element analysis , Modeling , Plates (structures) , Stiffness AND Soft tissues ,
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      In Vivo Model for Evaluating the Effects of Mechanical Stimulation on Tissue-Engineered Bone Repair

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    http://yetl.yabesh.ir/yetl1/handle/yetl/139884
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    • Journal of Biomechanical Engineering

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    contributor authorJoel D. Boerckel
    contributor authorYash M. Kolambkar
    contributor authorAngela S. P. Lin
    contributor authorRobert E. Guldberg
    contributor authorKenneth M. Dupont
    date accessioned2017-05-09T00:31:34Z
    date available2017-05-09T00:31:34Z
    date copyrightAugust, 2009
    date issued2009
    identifier issn0148-0731
    identifier otherJBENDY-27015#084502_1.pdf
    identifier urihttp://yetl.yabesh.ir/yetl/handle/yetl/139884
    description abstractIt has long been known that the bone adapts according to the local mechanical environment. To date, however, a model for studying the effects of functional mechanical loading on tissue-engineered bone repair in vivo has not yet been established. We have developed a rat femoral defect model, in which ambulatory loads are transduced through the implanted tissue-engineered construct to elucidate the role of the mechanical environment in functional restoration of a large bone defect. This model uses compliant fixation plates with integrated elastomeric segments, which allow transduction of ambulatory loads. Multiaxially and uniaxially compliant plates were characterized by mechanical testing and evaluated using in vivo pilot studies. In the first study, experimental limbs were implanted with multiaxial plates, which have a low stiffness in multiple loading modes. In the second study, experimental limbs were stabilized by a uniaxial plate, which allowed only axial deformation of the defect. X-ray scans and mechanical testing revealed that the multiaxial plates were insufficient to stabilize the defect and prevent fracture under ambulatory loads as a result of low flexural and torsional stiffness. The uniaxial plates, however, maintained integrity of the defect when implanted over a 12 week period. Postmortem microCT scans revealed a 19% increase in bone volume in the axially loaded limb compared with the contralateral standard control, and postmortem mechanical testing indicated that torsional strength and stiffness were increased 25.6- and 3.9-fold, respectively, compared with the control. Finite element modeling revealed high strain gradients in the soft tissue adjacent to the newly formed bone within the implanted construct. This study introduces an in vivo model for studying the effects of physiological mechanical loading on tissue-engineered bone repair. Preliminary results using this new in vivo model with the uniaxially compliant plate showed positive effects of load-bearing on functional defect repair.
    publisherThe American Society of Mechanical Engineers (ASME)
    titleIn Vivo Model for Evaluating the Effects of Mechanical Stimulation on Tissue-Engineered Bone Repair
    typeJournal Paper
    journal volume131
    journal issue8
    journal titleJournal of Biomechanical Engineering
    identifier doi10.1115/1.3148472
    journal fristpage84502
    identifier eissn1528-8951
    keywordsMaintenance
    keywordsStress
    keywordsBiological tissues
    keywordsBone
    keywordsFinite element analysis
    keywordsModeling
    keywordsPlates (structures)
    keywordsStiffness AND Soft tissues
    treeJournal of Biomechanical Engineering:;2009:;volume( 131 ):;issue: 008
    contenttypeFulltext
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