Modeling the Mechanics of Tethers Pulled From the Cochlear Outer Hair Cell MembraneSource: Journal of Biomechanical Engineering:;2008:;volume( 130 ):;issue: 003::page 31007Author:Kristopher R. Schumacher
,
Bahman Anvari
,
William E. Brownell
,
Alexander A. Spector
,
Aleksander S. Popel
DOI: 10.1115/1.2907758Publisher: The American Society of Mechanical Engineers (ASME)
Abstract: Cell membrane tethers are formed naturally (e.g., in leukocyte rolling) and experimentally to probe membrane properties. In cochlear outer hair cells, the plasma membrane is part of the trilayer lateral wall, where the membrane is attached to the cytoskeleton by a system of radial pillars. The mechanics of these cells is important to the sound amplification and frequency selectivity of the ear. We present a modeling study to simulate the membrane deflection, bending, and interaction with the cytoskeleton in the outer hair cell tether pulling experiment. In our analysis, three regions of the membrane are considered: the body of a cylindrical tether, the area where the membrane is attached and interacts with the cytoskeleton, and the transition region between the two. By using a computational method, we found the shape of the membrane in all three regions over a range of tether lengths and forces observed in experiments. We also analyze the effects of biophysical properties of the membrane, including the bending modulus and the forces of the membrane adhesion to the cytoskeleton. The model’s results provide a better understanding of the mechanics of tethers pulled from cell membranes.
keyword(s): Force , Columns (Structural) , Membranes , Plasmas (Ionized gases) AND Deflection ,
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contributor author | Kristopher R. Schumacher | |
contributor author | Bahman Anvari | |
contributor author | William E. Brownell | |
contributor author | Alexander A. Spector | |
contributor author | Aleksander S. Popel | |
date accessioned | 2017-05-09T00:27:00Z | |
date available | 2017-05-09T00:27:00Z | |
date copyright | June, 2008 | |
date issued | 2008 | |
identifier issn | 0148-0731 | |
identifier other | JBENDY-26808#031007_1.pdf | |
identifier uri | http://yetl.yabesh.ir/yetl/handle/yetl/137458 | |
description abstract | Cell membrane tethers are formed naturally (e.g., in leukocyte rolling) and experimentally to probe membrane properties. In cochlear outer hair cells, the plasma membrane is part of the trilayer lateral wall, where the membrane is attached to the cytoskeleton by a system of radial pillars. The mechanics of these cells is important to the sound amplification and frequency selectivity of the ear. We present a modeling study to simulate the membrane deflection, bending, and interaction with the cytoskeleton in the outer hair cell tether pulling experiment. In our analysis, three regions of the membrane are considered: the body of a cylindrical tether, the area where the membrane is attached and interacts with the cytoskeleton, and the transition region between the two. By using a computational method, we found the shape of the membrane in all three regions over a range of tether lengths and forces observed in experiments. We also analyze the effects of biophysical properties of the membrane, including the bending modulus and the forces of the membrane adhesion to the cytoskeleton. The model’s results provide a better understanding of the mechanics of tethers pulled from cell membranes. | |
publisher | The American Society of Mechanical Engineers (ASME) | |
title | Modeling the Mechanics of Tethers Pulled From the Cochlear Outer Hair Cell Membrane | |
type | Journal Paper | |
journal volume | 130 | |
journal issue | 3 | |
journal title | Journal of Biomechanical Engineering | |
identifier doi | 10.1115/1.2907758 | |
journal fristpage | 31007 | |
identifier eissn | 1528-8951 | |
keywords | Force | |
keywords | Columns (Structural) | |
keywords | Membranes | |
keywords | Plasmas (Ionized gases) AND Deflection | |
tree | Journal of Biomechanical Engineering:;2008:;volume( 130 ):;issue: 003 | |
contenttype | Fulltext |