Soluble Microbial Products in ABR Treating Low-Strength Wastewater

Abstract

The chemical composition, molecular weight (MW) distribution, and biodegradability (both aerobic and anaerobic) of soluble microbial products (SMPs) in an anaerobic baffled reactor (ABR) treating low-strength wastewater were investigated. The effect of various process parameters on the production of SMPs was also examined. Results indicated that high MW (>300 kDa) compounds were produced in the middle compartments of the reactor and formed 22% of the effluent chemical oxygen demand (COD). This fraction was found to be 86% degradable under aerobic conditions but only 4% under anaerobic conditions. Low MW (<1 kDa) material represented the highest portion (36%) of the effluent COD and was mainly found in the first compartment of the ABR and in the effluent. This fraction was more easily degraded under anaerobic conditions (33%) than aerobic conditions (17%). Analysis of a hydrolyzed sample of the high MW fraction revealed the presence of several sugars and volatile fatty acids. Therefore, it was concluded that the high MW material contains heteropolysaccharides. Nuclear magnetic resonance analysis of the low MW fraction revealed the possible presence of alcohol, carboxylate, and aromatic chemical groups. SMP production increased with increasing hydraulic retention time (HRT), probably due to enhanced biomass decay at high HRTs, and also increased with decreasing temperature, probably due to increased stress on the biomass and a reduced metabolism of the SMP at low temperatures. Finally, SMP production in an ABR containing higher levels of initial biomass concentration was greater than for an ABR operating at the same conditions but with lower levels of initial biomass.