| description abstract | Microbially induced calcium carbonate precipitation (MICP) is an emerging ecofriendly microbial engineering technique that utilizes urease-producing microorganisms to enhance the mechanical properties of soils. Sporosarcina pasteurii (S. pasteurii) stands out among these microorganisms as an efficient urease producer. However, field trials often lead to less-than-optimal experimental outcomes due to the presence of native soil microbes. To evaluate the impact of indigenous microorganisms on the effectiveness of MICP at the site, bacteria isolated from natural soil, classified of on-site low-ureolysis and high-ureolysis bacteria (OSLUB and OSHUB, respectively), were combined with S. pasteurii to conduct MICP experiments both in microfluidic chips and sand columns. Analysis covered the bacterial population, urease activity, pH changes, calcium carbonate crystal count and volume, as well as the unconfined compressive strength (UCS) of reinforced samples. Experimental results revealed that combining OSLUB with S. pasteurii led to a reduction in bacterial activity of 74% to 84% by 120 h, resulting in an approximately 60% decrease in the chemical conversion rate and the UCS of MICP-treated soils was 60% lower than the S. pasteurii. However, when OSHUB is mixed with S. pasteurii, although there is a reduction in bacterial activity by 49% to 54% by the 120-h mark, the decrease remains less pronounced than the activity decrease observed in S. pasteurii alone, which is 64%. Consequently, the rates of calcium carbonate chemical conversion were enhanced by 9% to 45%, and the UCS of the reinforced sand columns showed a slight improvement relative to the control group. This research highlights the distinct impacts of OSLUB and OSHUB on the efficiency of MICP on location. The main difference between OSLUB and OSHUB lies in their respective effects on pH levels following mixing. OSLUB tends to decrease the pH level gradually in the combined bacterial environment, while OSHUB, in contrast, increases the pH level over time in the same setting. The maintenance of both high bacterial activity and high precipitation rates is crucially dependent on pH levels, highlighting the importance of these findings for enhancing MICP efficiency in field applications. Strategies that either diminish the presence of OSLUB while augmenting that of OSHUB, or that sustain a relatively high pH level, could be valuable. These avenues promise significant improvements and merit further investigation in future studies. | |
