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contributor authorThakare, Ketan
contributor authorJerpseth, Laura
contributor authorPei, Zhijian
contributor authorQin, Hongmin
date accessioned2022-05-08T08:23:41Z
date available2022-05-08T08:23:41Z
date copyright4/8/2022 12:00:00 AM
date issued2022
identifier issn1087-1357
identifier othermanu_144_9_094502.pdf
identifier urihttp://yetl.yabesh.ir/yetl1/handle/yetl/4283874
description abstractThree-dimensional printing of hydrogel constructs containing algae cells, also known as green bioprinting, has many potential applications. Its feasibility has been demonstrated for removing metal contaminants in water and tissue engineering. However, in reported studies on green bioprinting so far, shape fidelity was either not investigated or poor. This paper reports a study to apply layer-by-layer photo-crosslinking in extrusion-based 3D printing of constructs using gelatin methacryloyl (GelMA) bioink containing Chlamydomonas reinhardtii algae cells. Shape fidelity and cell viability are compared between constructs printed by applying layer-by-layer photo-crosslinking and those printed by applying once-post-printing photo-crosslinking. Comparisons of shape fidelity are also made with alginate-methylcellulose constructs printed by applying ionic-crosslinking. Results show that GelMA constructs printed by applying layer-by-layer photo-crosslinking have the highest shape fidelity among all printed constructs, and about three-quarters of the algae cells in all printed GelMA constructs are alive four days post-printing. There are no significant differences in cell viability between the GelMA constructs printed by applying layer-by-layer photo-crosslinking and the GelMA constructs printed by applying once-post-printing photo-crosslinking.
publisherThe American Society of Mechanical Engineers (ASME)
titleApplying Layer-by-Layer Photo-Crosslinking in Green Bioprinting: Shape Fidelity and Cell Viability of Printed Hydrogel Constructs Containing Algae Cells
typeJournal Paper
journal volume144
journal issue9
journal titleJournal of Manufacturing Science and Engineering
identifier doi10.1115/1.4054086
journal fristpage94502-1
journal lastpage94502-8
page8
treeJournal of Manufacturing Science and Engineering:;2022:;volume( 144 ):;issue: 009
contenttypeFulltext


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