Systematic Approach for Modeling Tetrachloroethene Biodegradation

Abstract

The anaerobic biodegradation of tetrachloroethene (PCE) is a reasonably well understood process. Specific organisms capable of using PCE as an electron acceptor for growth require the addition of an electron donor to remove PCE from contaminated ground waters. However, competition from other anaerobic microorganisms for added electron donor will influence the rate and completeness of PCE degradation. The approach developed here allows for the explicit modeling of PCE and byproduct biodegradation as a function of electron donor and byproduct concentrations, and the microbiological ecology of the system. The approach is general and can be easily modified for ready use with in situ ground-water models or ex situ reactor models. Simulations conducted with models developed from this approach show the sensitivity of PCE biodegradation to input parameter values, in particular initial biomass concentrations. Additionally, the dechlorination rate will be strongly influenced by the microbial ecology of the system. Finally, comparison with experimental acclimation results indicates that existing kinetic constants may not be generally applicable. Better techniques for measuring the biomass of specific organism groups in mixed systems are required.